LOCUS       sequence-70029-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses HA tagged SH3BP4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
  TITLE     SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 
            signaling.
  JOURNAL   Mol Cell. 2012 Jun 29;46(6):833-46. doi: 
            10.1016/j.molcel.2012.04.007. Epub 2012 May 9.
  PUBMED    22575674
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.04.007"; journalName: "Mol Cell"; date: 
            "2012-06-29- 29"; volume: "46"; issue: "6"; pages: "833-46"
COMMENT     Sequence Label: pRK5-HA-SH3BP4
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtccactccc aggtccaact gcacctcggt tctatcgatt gaattcccga accgacagtc
       61 ggtctcttca ccaaggccat tcgcgccacc atgggctacc cctatgatgt gcctgactac
      121 gcagatctca atggtggtgg tggtgggtcg accatggcgg ctcagcggat ccgagcggcc
      181 aactccaatg gcctccctcg ctgcaagtca gaggggaccc tgattgacct gagcgaaggg
      241 ttttcagaga cgagctttaa tgacatcaaa gtgccttctc ccagtgcctt gctcgtagac
      301 aaccccacac ctttcggaaa tgcaaaggaa gtgattgcga tcaaggacta ttgccccacc
      361 aacttcacca cactgaagtt ctccaagggc gaccatctct acgtcttgga cacatctggc
      421 ggtgagtggt ggtacgcaca caacaccacc gaaatgggct acatcccctc ctcctatgtg
      481 cagcccttga actaccggaa ctcaacactg agtgacagcg gtatgattga taatcttcca
      541 gacagcccag acgaggtagc caaggagctg gagctgctcg ggggatggac agatgacaaa
      601 aaagtaccag gcagaatgta cagtaataac cctttctgga atggggtcca gaccaatcca
      661 tttctgaatg ggaacgtgcc cgtcatgccc agcctggatg agctgaatcc caaaagtact
      721 gtggatttgc tcctttttga cgcaggtaca tcctccttca ccgaatccag ctcagccacc
      781 acgaatagca ctggcaacat cttcgatgag cttccagtca caaacggact ccacgcagag
      841 ccgccggtca ggcgggacaa ccccttcttc agaagcaagc gctcctacag tctctcggaa
      901 ctctccgtcc tccaagccaa gtccgatgct ccacatcgtc
//
