LOCUS       sequence-70030-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Myc tagged SH3BP4 W92A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
  TITLE     SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 
            signaling.
  JOURNAL   Mol Cell. 2012 Jun 29;46(6):833-46. doi: 
            10.1016/j.molcel.2012.04.007. Epub 2012 May 9.
  PUBMED    22575674
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.04.007"; journalName: "Mol Cell"; date: 
            "2012-06-29- 29"; volume: "46"; issue: "6"; pages: "833-46"
COMMENT     Sequence Label: pRK5-Myc-SH3BP4 W92A
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcccgaa ccgacagtcg
       61 gtctcttcac caaggccatt cgcgccacca tggagcaaaa gctcatttct gaggaagatc
      121 tcaatggtgg tggtggtggg tcgaccatgg cggctcagcg gatccgagcg gccaactcca
      181 atggcctccc tcgctgcaag tcagagggga ccctgattga cctgagcgaa gggttttcag
      241 agacgagctt taatgacatc aaagtgcctt ctcccagtgc cttgctcgta gacaacccca
      301 cacctttcgg aaatgcaaag gaagtgattg cgatcaagga ctattgcccc accaacttca
      361 ccacactgaa gttctccaag ggcgaccatc tctacgtctt ggacacatct ggcggtgagg
      421 cgtggtacgc acacaacacc accgaaatgg gctacatccc ctcctcctat gtgcagccct
      481 tgaactaccg gaactcaaca ctgagtgaca gcggtatgat tgataatctt ccagacagcc
      541 cagacgaggt agccaaggag ctggagctgc tcgggggatg gacagatgac aaaaaagtac
      601 caggcagaat gtacagtaat aaccctttct ggaatggggt ccagaccaat ccatttctga
      661 atgggaacgt gcccgtcatg cccagcctgg atgagctgaa tcccaaaagt actgtggatt
      721 tgctcctttt tgacgcaggt acatcctcct tcaccgaatc cagctcagcc accacgaata
      781 gcactggcaa catcttcgat gagcttccag tcacaaacgg actccacgca gagccgccgg
      841 tcaggcggga caaccccttc ttcagaagca agcgctccta cagtctctcg gaactctccg
      901 tcctccaagc caagtc
//
