LOCUS       sequence-70031-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses HA tagged SH3BP4 W92A mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Kim YM, Stone M, Hwang TH, Kim YG, Dunlevy JR, Griffin TJ, Kim DH
  TITLE     SH3BP4 is a negative regulator of amino acid-Rag GTPase-mTORC1 
            signaling.
  JOURNAL   Mol Cell. 2012 Jun 29;46(6):833-46. doi: 
            10.1016/j.molcel.2012.04.007. Epub 2012 May 9.
  PUBMED    22575674
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.04.007"; journalName: "Mol Cell"; date: 
            "2012-06-29- 29"; volume: "46"; issue: "6"; pages: "833-46"
COMMENT     Sequence Label: pRK5-HA-SH3BP4 W92A
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      151..160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcccgaa ccgacagtcg
       61 gtctcttcac caaggccatt cgcgccacca tgggctaccc ctatgatgtg cctgactacg
      121 cagatctcaa tggtggtggt ggtgggtcga ccatggcggc tcagcggatc cgagcggcca
      181 actccaatgg cctccctcgc tgcaagtcag aggggaccct gattgacctg agcgaagggt
      241 tttcagagac gagctttaat gacatcaaag tgccttctcc cagtgccttg ctcgtagaca
      301 accccacacc tttcggaaat gcaaaggaag tgattgcgat caaggactat tgccccacca
      361 acttcaccac actgaagttc tccaagggcg accatctcta cgtcttggac acatctggcg
      421 gtgaggcgtg gtacgcacac aacaccaccg aaatgggcta catcccctcc tcctatgtgc
      481 agcccttgaa ctaccggaac tcaacactga gtgacagcgg tatgattgat aatcttccag
      541 acagcccaga cgaggtagcc aaggagctgg agctgctcgg gggatggaca gatgacaaaa
      601 aagtaccagg cagaatgtac agtaataacc ctttctggaa tggggtccag accaatccat
      661 ttctgaatgg gaacgtgccc gtcatgccca gcctggatga gctgaatccc aaaagtactg
      721 tggatttgct cctttttgac gcaggtacat cctccttcac cgaatccagc tcagccacca
      781 cgaatagcac tggcaacatc ttcgatgagc ttccagtcac aaacggactc cacgcagagc
      841 cgccggtcag gcgggacaac cccttcttca gaagcaagcg ctcctacagt ctctcggaac
      901 tctccgtcct ccaagccaag tccgatgctc ccacatcgtc gagtttcttc accggcttga
      961 aa
//
