LOCUS       sequence-70036-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-8 (seed1-8)
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(220..319)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(336..355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(337..355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(360..376)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(360..376)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(478..497)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(556..575)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gataacacgt tggctctacc
       61 tcatctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      121 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      181 ttattgcagc ttataatggt tacaagggtg ggcgcgccga cccagctttc ttgtacaaag
      241 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
      301 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
      361 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
      421 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
      481 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
      541 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
      601 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
      661 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
      721 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
      781 cgatccccgg aaaaacagca ttccaggtat tagaagaata t
//
