LOCUS       sequence-70037-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-9 (bulge 9-11)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(222..321)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(338..357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(339..357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(362..378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(362..378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(480..499)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(558..577)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tcggcatgga cgagctgtac cagtaaagcg gccgcgactc tagaactata caacgatcta
       61 cctcatctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
      121 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      181 gtttattgca gcttataatg gttacaaggg tgggcgcgcc gacccagctt tcttgtacaa
      241 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      301 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
      361 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
      421 acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac
      481 aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat
      541 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      601 aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      661 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      721 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
      781 tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtg
//
