LOCUS       sequence-70048-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag, Myc tagged mutant Nbr in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL
  TITLE     The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs
            bound to Drosophila Argonaute1.
  JOURNAL   Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.
  PUBMED    22055293
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2011-11-22"; volume: "21(22)"; pages: "1878-87. Epub 
            2011 Nov 3"
COMMENT     Sequence Label: pAFMW-Nbr-D435A/E437A
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      106..115
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             115..180
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      196..205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             205..234
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             244..273
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             283..312
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             322..351
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             361..390
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             424..453
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    466..490
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggatccagat atcgaggcct gtctagagaa gcttgttcga atctcgagtg cgcgcttccg
       61 gaggtataca cctaggcggt accactgcag tgaattcgga gctccgccac catggactac
      121 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
      181 caccggttga gctccgccac catggagcaa aagctcattt ctgaagagga cttgaatgaa
      241 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct
      301 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      361 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg cgacctcacc
      421 atggagcaaa agctcatttc tgaagaggac ttgaatcacc ggtatacaag tttgtacaaa
      481 aaagcaggct ccgcggccgc ccccttcacc atggcacgca agagccacat gtacaacgca
      541 atacccgccg gctttgagtc ggacgaggag aacatggaga acctaatgag caacctgaag
      601 atcaagcgct tggaggacat cacgacaggt gctggaatcg acggctgtaa ctttgacgcc
      661 accttggacg caaaggcgga ggagtttttt aagctgttcc gcgagaagtg gaacatgtat
      721 agcaagaaaa agtcgccaca tctgcgccag gaatttggaa gggctttaat gggccatcag
      781 gatcctttgc tcctggccct gaagatcttc gccaactgcc cagacagcag caacatcaag
      841 acgaagagct tgtcacattt tgtgctggac acggtttgca gctccacaag gatttcccgc
//
