LOCUS       sequence-70054-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag, Myc tagged mutant Nbr in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL
  TITLE     The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs
            bound to Drosophila Argonaute1.
  JOURNAL   Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.
  PUBMED    22055293
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2011-11-22"; volume: "21(22)"; pages: "1878-87. Epub 
            2011 Nov 3"
COMMENT     Sequence Label: pAFMW-Nbr-D435A/E437A
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          121..186
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    278..302
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg agcaaaacag
       61 gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat aggttggaat
      121 ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta aaaattttat
      181 atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca caccacagaa
      241 gtaaggttcc ttcacaaaga tcctgctagc ttacgtcacc actttgtaca agaaagctgg
      301 gtcggcgcgc ccaccctttc acttaacatg ggcaccccgc ctgagaaagt tattgctggc
      361 gatgctcttt tcgataaccg cttgtatgaa ggacacgcgt tcaatgagcg tgttgtagat
      421 cagcatcaaa caccgcgcat cgatggctgc ataaagaatc tgctcgcgac gcagcggtct
      481 attggcccag tttgagcact ggtttgattt gttcagtttc ttgccaaggc atgccaatga
      541 gagatccgta agggcgtctc ctgcccggtt aacattgccg aagggaagtt caacgccgaa
      601 gcgctgcttt tttaactcca accagagatt gcgaaggtcc aagtagtggt gaggcatttg
      661 aaggcgcagt tgcaggggca gtgaccgctg caatacactg agatcactga ccatagagaa
      721 ccccacctta cggatgttca cgttattaaa gatattcgcg cccagcaagc gccagtgttc
      781 cgagcgtaag ctctcccgcg ccaggcaatc gatcaggtag acattgtggc cggtggcgat
      841 ctgtagcacg cacagctgat tgtctccgca cacgctctgc atccaggcag aggccaggta
      901 tatgacgcac tcctgctgca gatggtataa catacgatcg aactcatcag c
//
