LOCUS       sequence-70056-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag, Myc tagged Nbr in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Han BW, Hung JH, Weng Z, Zamore PD, Ameres SL
  TITLE     The 3'-to-5' exoribonuclease Nibbler shapes the 3' ends of microRNAs
            bound to Drosophila Argonaute1.
  JOURNAL   Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.
  PUBMED    22055293
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2011-11-22"; volume: "21(22)"; pages: "1878-87. Epub 
            2011 Nov 3"
COMMENT     Sequence Label: pAFM-NbrWT
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             116..181
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..235
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             245..274
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             284..313
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             323..352
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             362..391
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             425..454
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    467..491
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggatccaga tatcgaggcc tgtctagaga agcttgttcg aatctcgagt gcgcgcttcc
       61 ggaggtatac acctaggcgg taccactgca gtgaattcgg agctccgcca ccatggacta
      121 caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
      181 gcaccggttg agctccgcca ccatggagca aaagctcatt tctgaagagg acttgaatga
      241 aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa agctcatttc
      301 tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact tgaatgaaat
      361 ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagagcttgg gcgacctcac
      421 catggagcaa aagctcattt ctgaagagga cttgaatcac cggtatacaa gtttgtacaa
      481 aaaagcaggc tccgcggccg cccccttcac catggcacgc aagagccaca tgtacaacgc
      541 aatacccgcc ggctttgagt cggacgagga gaacatggag aacctaatga gcaacctgaa
      601 gatcaagcgc ttggaggaca tcacgacagg tgctggaatc gacggctgta actttgacgc
      661 caccttggac gcaaaggcgg aggagttttt taagctgttc cgcgagaagt ggaacatgta
      721 tagcaagaaa aagtcgccac atctgcgcca ggaatttgga agggctttaa tgggccatca
      781 ggatcctttg ctcctggccc tgaagatctt cgccaactgc ccagacagca gcaacatcaa
      841 gacgaagagc ttgtcacatt ttgtgctgga cacggtttgc aagctccaca aggattttcc
//
