LOCUS       sequence-70060-e        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Defective for E6AP binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 16E6no* I128T
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(788..807)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    853..908
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(907..926)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
       61 ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg
      121 tgctgtctca tcattttggc aaagaattcc tcgacggatc tatggactac aaggatgacg
      181 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgat atcggatcct
      241 ttcaggaccc acaggagcga cccagaaagt taccacagtt atgcacagag ctgcaaacaa
      301 ctatacatga tataatatta gaatgtgtgt actgcaagca acagttactg cgacgcgagc
      361 tatatgactt tgcttttcgg gatttatgca tagtatatag agatgggaat ccatatgctg
      421 tatgtgataa atgtttaaag ttttattcta aaattagtga gtatagacat tattgttata
      481 gtttgtatgg aacaacatta gaacagcaat acaacaaacc gttgtgtgat ttgttaatta
      541 ggtgtattaa ctgtcaaaag ccactgtgtc ctgaagaaaa gcaaagacat ctggacaaaa
      601 agcaaagatt ccataataca aggggtcggt ggaccggtcg atgtatgtct tgttgcagat
      661 catcaagaac acgtagagaa acccagctgt aaagatccgt cgaggaattc actcctcagg
      721 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
      781 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
      841 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
      901 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
      961 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     1021 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     1081 ccttgacttg agg
//
