LOCUS       sequence-70062-e         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRNA sensor used in Drosophila cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAW-EGFP-2xmiR-1 (pAW-AS-21)
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(242..266)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          358..423
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             553..573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acgagctgta caagtaaagc ggccgcgact ctagactcca tacttcttta cattccatct
       61 agactccata cttctttaca ttccatctag atcataatca gccataccac atttgtagag
      121 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      181 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaggg tgggcgcgcc
      241 gacccagctt tcttgtacaa agtggtgacg taagctagca ggatctttgt gaaggaacct
      301 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
      361 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
      421 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
      481 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
      541 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
      601 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
      661 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
      721 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
      781 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
      841 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
      901 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
      961 gaacctgaaa cataaaatga atgcaat
//
