LOCUS       sequence-70064-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRNA sensor used in Drosophila cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAW-GFP-2xmiR-34 (pAW-AS-25)
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(244..268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          360..425
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             555..575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctgtaca agtaaagcgg ccgcgactct agacacaacc agctaaccac actgccatct
       61 agacacaacc agctaaccac actgccatct agatcataat cagccatacc acatttgtag
      121 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      181 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaag ggtgggcgcg
      241 ccgacccagc tttcttgtac aaagtggtga cgtaagctag caggatcttt gtgaaggaac
      301 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      361 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
      421 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
      481 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
      541 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
      601 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
      661 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
      721 taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
      781 acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
      841 taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
      901 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      961 ctgaacctga aacataaatg aatgca
//
