LOCUS       sequence-70065-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  miRNA sensor used in Drosophila cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAW-EGFP-4xlet-7 (pAW-AS27)
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(305..329)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          421..486
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             616..636
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagaacta tacaacctac
       61 tacctcatct agaactatac aacctactac ctcatctaga actatacaac ctactacctc
      121 atctagaact atacaaccta ctacctcatc tagatcataa tcagccatac cacatttgta
      181 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      241 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa gggtgggcgc
      301 gccgacccag ctttcttgta caaagtggtg acgtaagcta gcaggatctt tgtgaaggaa
      361 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
      421 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
      481 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
      541 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
      601 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
      661 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
      721 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
      781 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
      841 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
      901 ttaatttgta aaggggtaat aaggaaatat ttgatgtata gtgccttgac tagagatcat
      961 aatcagccat accacatt
//
