LOCUS       sequence-70324-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Target RNAs used to determine complementarity requirements for in 
            vitro tailing and degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Ameres SL, Horwich MD, Hung JH, Xu J, Ghildiyal M, Weng Z, Zamore PD
  TITLE     Target RNA-directed trimming and tailing of small silencing RNAs.
  JOURNAL   Science. 2010 Jun 18;328(5985):1534-9.
  PUBMED    20558712
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2010-06-18"; volume: "328(5985)"; pages: "1534-9"
COMMENT     Sequence Label: pAS-1 (perf)
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(213..312)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(329..348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(330..348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(353..369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(353..369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(471..490)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(549..568)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 acgagctgta caagtaaagc ggccgcgact ctagaactat acaacctact acctcatcta
       61 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      121 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      181 agcttataat ggttacaagg gtgggcgcgc cgacccagct ttcttgtaca aagttggcat
      241 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
      301 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
      361 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
      421 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
      481 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
      541 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
      601 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
      661 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
      721 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
      781 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
      841 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
      901 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaa
//
