LOCUS       sequence-70334-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+D839A+H840A+N863A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m4
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagattatcg aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt
       61 tttcattttc ttgacaactt cttctgaggg gacgttatca ctcttccctc tagctttatc
      121 ggatcttgtc aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat
      181 agcagccacg tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc
      241 cctgccgttc tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac
      301 tgggtgttcc ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat
      361 cctttcccta ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat
      421 aacgatattc tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac
      481 ggtctgcagt attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg
      541 aagactgtcc ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga
      601 gtcatcatgg atcaactgca tgaagttccg gttggcaaat ccatcggact taagaaaatc
      661 caggattgtc tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg
      721 cccccatcct gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cgaagagatg
      781 agcgtaagtt ttcaagcgtt cttcaatcat ctccctatct tcaaacaacg taagggtgag
      841 gacaatgtcc tcaagaatgt cctcgttctc ctcattgtcc aggaagtcct tgtctttaat
      901 gattttcagg agatcgtgat acgttcccag ggatgcgttg aagcgatcct ccactccgct
      961 gatttcaaca gagtcgaaac attcaatctt tttgaaatag tcttctttga
//
