LOCUS       sequence-70337-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+H840A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m2
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
       61 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
      121 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatagcat
      181 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
      241 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
      301 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
      361 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
      421 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
      481 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtgaagac
      541 tgtccccctg gccagaaact tgtgctttct ggatgtcctc cttaaaggtg agagagtcat
      601 catggatcaa ctgcatgaag ttccggttgg caaatccatc ggacttaaga aaatccagga
      661 ttgtctttcc actctgcttg tctcggatcc cattgatcag ttttcttgac agccgccccc
      721 atcctgtata tcggcgcctc ttgagctgtt tcatgacttt gtcgtcgaag agatgagcgt
      781 aagttttcaa gcgttcttca atcatctccc tatcttcaaa caacgtaagg gtgaggacaa
      841 tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa gtccttgtct ttaatgattt
      901 tcaggagatc gtgatacgtt cccagggatg cgttgaagcg atcctccact ccgctgattt
      961 caacagagtc gaaacattca atctttttga aata
//
