LOCUS       sequence-70341-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  General cloning vector for use in E. coli or D. radiodurans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Meima R, Lidstrom ME
  TITLE     Characterization of the minimal replicon of a cryptic Deinococcus 
            radiodurans SARK plasmid and development of versatile Escherichia 
            coli-D. radiodurans shuttle vectors.
  JOURNAL   Appl Environ Microbiol. 2000 Sep;66(9):3856-67.
  PUBMED    10966401
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2000-09"; volume: "66(9)"; pages: 
            "3856-67"
COMMENT     Sequence Label: pRAD1
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     207..224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    341..362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(377..382,383..400,401..407)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    415..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     420..442
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     439..455
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     439..455
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      121 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      181 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      241 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      301 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      361 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      421 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgcgaggc
      481 ctcgagatct atcgatgcat gccatggtac ccgggagctc gaattctaga agcttctgca
      541 gacgcgtcga cgtcatatgg atccgattcg acctgcaggc atgcaagctg tacgcctcag
      601 ctaccattct tgcagccgcg ttcagggtct tgacgtgttc tttgtatcta tcagcaaact
      661 gagaacccaa ttctggatca tccttgtaac gccaactctc cgaccattcc gaaggaacgc
      721 cttgtagtcc tcctgagccg ttagggtatg tctttagtcc aatcaaatgg gacatttgca
      781 gacaggaacg cacaagtgtc tcacgtcgct tctctatttc aatatctaaa aattcttcac
      841 tcttatcgtt gcttgacata ttaaaactgt ctaactgatc cagttcactt atatggaaag
      901 tatcactaaa gtcatggctt ctcagaaatt caatacttcc cattgatggg gaccggttaa
      961 ccaaatcgat aaaaattttt ctgtcatgtt tcagataagt ttttggcgcg ttgattttat
     1021 aataaagccg ccattct
//
