LOCUS       sequence-70342-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, 
            Devenney PS, Hohenstein P
  TITLE     A universal vector for high-efficiency multi-fragment recombineering
            of BACs and knock-in constructs.
  JOURNAL   PLoS One. 2013 Apr 29;8(4):e62054. doi: 
            10.1371/journal.pone.0062054. Print 2013.
  PUBMED    23637962
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-04-29"; volume: "8(4):e62054. doi"; pages: " 
            10.1371/journal.pone.0062054. Print 2013"
COMMENT     Sequence Label: pENTR eGFPCRE L1-R5
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..173)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgcggccgcc ctgcagtaca ggtcactaat accatctaag tagttgattc atagtgactg
       61 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      121 attgatattt atatcatttt acgtttctcg ttcaactttt gtatacaaag ttgtctaatc
      181 gccatcttcc agcaggcgca ccattgcccc tgtttcacta tccaggttac ggatatagtt
      241 catgacaata tttacattgg tccagccacc agcttgcatg atctccggta ttgaaactcc
      301 agcgcgggcc atatctcgcg cggctccgac acgggcactg tgtccagacc aggccaggta
      361 tctctgacca gagtcatcct tagcgccgta aatcaatcga tgagttgctt caaaaatccc
      421 ttccagggcg cgagttgata gctggctggt ggcagatggc gcggcaacac cattttttct
      481 gacccggcaa aacaggtagt tattcggatc atcagctaca ccagagacgg aaatccatcg
      541 ctcgaccagt ttagttaccc ccaggctaag tgccttctct acacctgcgg tgctaaccag
      601 cgttttcgtt ctgccaatat ggattaacat tctcccaccg tcagtacgtg agatatcttt
      661 aaccctgatc ctggcaattt cggctatacg taacagggtg ttataagcaa tccccagaaa
      721 tgccagatta cgtatatcct ggcagcgatc gctattttcc atgagtgaac gaacctggtc
      781 gaaatcagtg cgttcgaacg ctagagcctg ttttgcacgt tcaccggcat caacgttttc
      841 ttttcggatc c
//
