LOCUS       sequence-70344-e        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  General cloning vector for use in E. coli or D. radiodurans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Meima R, Lidstrom ME
  TITLE     Characterization of the minimal replicon of a cryptic Deinococcus 
            radiodurans SARK plasmid and development of versatile Escherichia 
            coli-D. radiodurans shuttle vectors.
  JOURNAL   Appl Environ Microbiol. 2000 Sep;66(9):3856-67.
  PUBMED    10966401
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2000-09"; volume: "66(9)"; pages: 
            "3856-67"
COMMENT     Sequence Label: pRAD1
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(169..957)
                     /codon_start=1
                     /gene="bla(M)"
                     /product="beta-lactamase lacking the signal sequence"
                     /label=bla(M)
                     /note="allows cytosolic expression of beta-lactamase"
                     /translation="PETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMST
                     FKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMS
                     DNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATT
                     LRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAA
                     LGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     792..811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgt
//
