LOCUS       sequence-70351-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  TITLE     Birnbaum lab plasmids
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAMPK alpha1 full length
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             146..166
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttgg taccatgggg tactaccatc accatcacca
      121 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tggccgagaa
      181 gcagaaacac gacgggcggg tgaagatcgg ccactacatc ctgggggaca cgcttggtgt
      241 cggcaccttc gggaaagtga aggtgggcaa gcacgagttg accggacata aagtggctgt
      301 gaagatactc aaccggcaga agattcggag ccttgacgtg gtgggaaaaa tccgccggga
      361 gattcagaac ctgaagctgt tcaggcaccc tcacatcatc aaactgtacc aggtcatcag
      421 tacaccatct gatattttca tggtgatgga atatgtctct ggaggagagc tatttgatta
      481 tatctgtaaa aatggaaggt tggacgaaaa ggaaagccgc cgtctgttcc agcagatcct
      541 ttccggtgtg gattattgtc acaggcatat ggtggtccac agagatttga aacctgagaa
      601 cgtcctgctt gatgcacaca tgaatgcaaa gatagccgac tttggtcttt caaacatgat
      661 gtcagatggt gaatttttaa gaacaagctg tggctcaccc aattatgccg caccagaagt
      721 catttcagga agattgtacg caggccccga ggtggacatc tggagcagcg gggtcattct
      781 ctatgctttg ctgtgtggaa ccctcccttt tgatgatgac catgtgccaa ctctttttaa
      841 gaagatatgt gatgggatct tttatacccc tcagtactta aacccttcag taatcagcct
      901 tttgaaacat atgctgcagg tgga
//
