LOCUS       sequence-70352-e        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Defective for E6AP binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 11E6 L111Q
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(796..815)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    861..916
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(915..934)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
       61 ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg
      121 tgctgtctca tcattttggc aaagaattcc tcgacggatc tatggactac aaggatgacg
      181 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgat tcgactggaa
      241 tggaaagtaa agatgcctcc acgtctgcaa catctataga ccagttgtgc aagacgttta
      301 atctttcttt gcacactctg caaattcagt gcgtgttttg caggaatgca ctgaccaccg
      361 cagagatata tgcatatgcc tataagaacc taaaggttgt gtggcgagac aactttccct
      421 ttgcagcgtg tgcctgttgc ttagaactgc aagggaaaat taaccaatat agacacttta
      481 attatgctgc atatgcacct acagtagaag aagaaaccaa tgaagatatt ttaaaagtgt
      541 taattcgttg ttacctgtgt cacaagccgc agtgtgaaat agaaaaacta aagcacatat
      601 tgggaaaggc acgcttcata aaactaaata accagtggaa gggtcgttgc ttacactgct
      661 ggacaacatg catggaagac ttgttaccct aaaagctatc ggatccgtcg aggaattcac
      721 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      781 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      841 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      901 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
      961 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     1021 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     1081 tagaaaagcc ttgacttgag gttagatttt ttttaaattt tgttttgggg tatttttttc
     1141 ttta
//
