LOCUS       sequence-70354-e        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a 
            T7 promoter for in vitro transcription, germline-compatible 5'UTR, 
            tbb-2 3'UTR and a polyA sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Katic I, Grosshans H
  TITLE     Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979578
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: pIK86
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..41
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     25..41
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        60..78
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgcgaatgca tctagatatc ggttgtaaaa cgacggccag tgaattcgag ctcggtacct
       61 aatacgactc actataggaa tacacggaat tctagatgat ccccgcgtac cgagctcaga
      121 aaaaatggat aaaaagtaca gtattggact ggatattgga acaaattcgg tgggatgggc
      181 cgtgattaca gatgagtata aggtgccaag taagaagttc aaggttcttg gaaacactga
      241 tcgtcattca attaaaaaga acttgatcgg agctcttttg ttcgattctg gagaaactgc
      301 agaggccacc cgtcttaaga gaaccgcaag acgacgttac acaagacgaa agaacagaat
      361 ctgttacttg caagaaatct tttcaaatga gatggctaaa gtcgatgata gtttctttca
      421 tcgattggaa gagtccttcc tcgttgaaga ggataaaaag catgaacgtc acccaatttt
      481 tggaaatatc gtggatgaag tcgcttacca tgagaaatat ccaacaattt accacctgcg
      541 taaaaagctt gttgattcta ctgataaggc agatctcaga ctgatttacc ttgctttggc
      601 acatatgatc aaattccgag gacactttct gattgaagga gatcttaatc cagataactc
      661 agatgtcgat aaattgttca ttcaactcgt tcagacttac aaccaacttt tcgaagagaa
      721 cccaatcaac gcctcgggag ttgatgccaa ggctattctg agtgctagac tttcgaaaag
      781 tcgtagactc gaaaatctga ttgcacagtt gccaggagag aaaaagaatg gattgtttgg
      841 aaacctcatt gccctctccc tgggacttac cccaaacttc aagtcaaact tcgatcttgc
      901 tgaagatgca aagttgcaac tctctaagga tacatacgat gatgatttgg ataatctcct
      961 ggcacagatt ggagatcaat atgccgatct gttccttgct gcaaaaaacc tttcgga
//
