LOCUS       sequence-70355-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid separately encodes a human codon-optimized SpCas9, a 
            tracrRNA and customizable crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: 
            pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..58)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt
       61 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      121 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      181 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      241 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
      301 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      361 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      421 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      481 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      541 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      601 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      661 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      721 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
      781 ggtcccagtc cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact
      841 ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac gatattcact tggggcatgc
      901 tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc ccacacgatc tccccggttt
      961 cgccgtttgt ctcgatca
//
