LOCUS       sequence-70361-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a a272g mutation in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-D91G
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(44..115)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acgcagaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca
       61 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      121 tggggatcct cactaccttg ctgctgacgc tgccggtctg ataagatcca gttctacacc
      181 cagtctgata gcctgtcggg cgttactgac gcccagtttc acgaccacat tcttgatgtg
      241 aaacttcacg gtactcacag aaatgcccgt aatagcggca atctcagcat aggttttgcc
      301 catactcgcc cagtacaaca cctcattttc acgcgaggaa aatatcgttt tgtccgcgct
      361 ctgatttaac gccggggctc gctcgccttc ggtgcctgcc agtcggtaca tctgctcgtt
      421 aaaatcaatc agcagcatct gcatcgtgcc ctgttcggca gcaaggcgtt gctccagcgc
      481 agtctgatcg ttgcctttaa tgatcacgga caacagagca aggttgttca tgtggtcatg
      541 caggacatag gtaaagccgt taacgatgtt gtattgcttg gataaagaga aaattttggt
      601 gaaccgcagg ccggacatca gcgtaatatt ctcatcccag gcaaacggcg aggtgcgttt
      661 aaaggccgtg agaataacgg gatcggtcag ctgaaagttg ttagcgcggt ataacctaat
      721 ccattcgtca ggataactgg aaataatcag aacatttgaa ggattttttt tgctcacaac
      781 agtgtaagcg taatccggac tacccagcgg agataacttt ctctgt
//
