LOCUS       sequence-70364-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a272g and c659t mutations in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-D91G/V220A
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(37..108)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
       61 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggggat
      121 cctcactacc ttgctgctga cgctgccggt ctgataagat ccagttctac acccagtctg
      181 atagcctgtc gggcgttact gacgcccagt ttcacggcca cattcttgat gtgaaacttc
      241 acggtactca cagaaatgcc cgtaatagcg gcaatctcag cataggtttt gcccatactc
      301 gcccagtaca acacctcatt ttcacgcgag gaaaatatcg ttttgtccgc gctctgattt
      361 aacgccgggg ctcgctcgcc ttcggtgcct gccagtcggt acatctgctc gttaaaatca
      421 atcagcagca tctgcatcgt gccctgttcg gcagcaaggc gttgctccag cgcagtctga
      481 tcgttgcctt taatgatcac ggacaacaga gcaaggttgt tcatgtggtc atgcaggaca
      541 taggtaaagc cgttaacgat gttgtattgc ttggataaag agaaaatttt ggtgaaccgc
      601 aggccggaca tcagcgtaat attctcatcc caggcaaacg gcgaggtgcg tttaaaggcc
      661 gtgagaataa cgggatcggt cagctgaaag ttgttagcgc ggtataacct aatccattcg
      721 tcaggataac tggaaataat cagaacattt gaaggatttt ttttgctcac aacagtgtaa
      781 gcgtaatccg gactacccag cggagata
//
