LOCUS       sequence-70365-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Defective for E6AP binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 18E6no* I130T
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(820..839)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    885..940
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(939..958)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
       61 ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg
      121 tgctgtctca tcattttggc aaagaattcc tcgacggatc tatggactac aaggatgacg
      181 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgat tcgactggaa
      241 tggcgcgctt tgaggatcca acacggcgac cctacaagct acctgatctg tgcacggaac
      301 tgaacacttc actgcaagac atagaaataa cctgtgtata ttgcaagaca gtattggaac
      361 ttacagaggc atttgaattt gcatttaaag atttatttgt ggtgtataga gacagtatac
      421 cgcatgctgc atgccataaa tgtatagatt tttattctag aattagagaa ttaagacatt
      481 attcagactc tgtgtatgga gacacattgg aaaaactaac taacactggg ttatacaatt
      541 tattaataag gtgcctgcgg tgccagaaac cgttgaatcc agcagaaaaa cttagacacc
      601 ttaatgaaaa acgacgattc cacaacacag ctgggcacta tagaggccag tgccattcgt
      661 gctgcaaccg agcacgacag gaacgactcc aacgacgcag agaaacacaa gtataaaagc
      721 tatcggatcc gtcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
      781 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
      841 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
      901 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
      961 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     1021 tatgctggct gccatgaaca aaggttggc
//
