LOCUS       sequence-70373-e        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tetracycline-inducible expression of human Flag tagged H3F3A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Huang C, Zhang Z, Xu M, Li Y, Li Z, Ma Y, Cai T, Zhu B
  TITLE     H3.3-H4 tetramer splitting events feature cell-type specific 
            enhancers.
  JOURNAL   PLoS Genet. 2013 Jun;9(6):e1003558. doi: 
            10.1371/journal.pgen.1003558. Epub 2013 Jun 6.
  PUBMED    23754967
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2013-06"; volume: "9(6):e1003558. doi"; pages: " 
            10.1371/journal.pgen.1003558. Epub 2013 Jun 6"
COMMENT     Sequence Label: pcDNA4/TO-Flag-H3.3
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    30..48
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     58..82
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             194..217
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(696..713)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    702..926
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
       61 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      121 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga
      181 gctcggatcc atggactaca aagacgatga cgacaaggct cgtacaaagc agactgcccg
      241 caaatcgacc ggtggtaaag cacccaggaa gcaactggct acaaaagccg ctcgcaagag
      301 tgcgccctct actggagggg tgaagaaacc tcatcgttac aggcctggta ctgtggcgct
      361 ccgtgaaatt agacgttatc agaagtccac tgaacttctg attcgcaaac ttcccttcca
      421 gcgtctggtg cgagaaattg ctcaggactt taaaacagat ctgcgcttcc agagcgcagc
      481 tatcggtgct ttgcaggagg caagcgaggc ctatctggtt ggcctttttg aagacaccaa
      541 cctgtgtgct atccatgcca aacgtgtaac aattatgcca aaagacatcc agctagcacg
      601 ccgcatacgt ggagaacgtg cttaagaatt ctgcagatat ccagcacagt ggcggccgct
      661 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
      721 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
      781 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
      841 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      901 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      961 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggt
//
