LOCUS       sequence-70384-e         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Smyth JW, Hong TT, Gao D, Vogan JM, Jensen BC, Fong TS, Simpson PC, 
            Stainier DY, Chi NC, Shaw RM
  TITLE     Limited forward trafficking of connexin 43 reduces cell-cell 
            coupling in stressed human and mouse myocardium.
  JOURNAL   J Clin Invest. 2010 Jan 4. 120(1):266-79.
  PUBMED    20038810
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2010 Jan 4. 120(1):266-79."
COMMENT     Sequence Label: plenti6.3/hCx43-stop
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             81..314
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(401..799)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(818..865)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 ataacagttc ctctcactct ctgatattca tttctttgca agttataaat actgaataat
       61 aagatgacat gaactactac tgctagagat tttccacact gactaaaagg gtctgaggga
      121 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
      181 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
      241 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
      301 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
      361 gtaagtcatt ggtcttaaag gtaccgagct cgaattgtgc ttagccctcc cacacataac
      421 cagagggcag caattcacga atcccaactg ccgtcggctg tccatcactg tccttcacta
      481 tggctttgat cccaggatgc agatcgagaa gcacctgtcg gcaccgtccg caggggctca
      541 agatgcccct gttctcattt ccgatcgcga cgatacaagt caggttgcca gctgccgcag
      601 cagcagcagt gcccagcacc acgagttctg cacaaggtcc cccagtaaaa tgatatacat
      661 tgacaccagt gaagatgcgg ccgtcgctag agagagctgc gctggcgacg ctgtagtctt
      721 cagagatggg gatgctgttg attgtagccg ttgctctttc aatgagggtg gattcttctt
      781 gagacaaagg cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat
      841 actatgccga tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc
      901 tcccgggagc tttttgcaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaa
//
