LOCUS       sequence-70391-e        6805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ozturk N, VanVickle-Chavez SJ, Akileswaran L, Van Gelder RN, Sancar 
            A
  TITLE     Ramshackle (Brwd3) promotes light-induced ubiquitylation of 
            Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4980-5. doi: 
            10.1073/pnas.1303234110. Epub 2013 Mar 11.
  PUBMED    23479607
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1303234110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "4980-5"
COMMENT     Sequence Label: pcDNA3.BRWD3-V5H
FEATURES             Location/Qualifiers
     source          1..6805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1286..1295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6730..6771
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6781..6798
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaaacta gacaacccag ttccggtgat gatctaaagc cagagctgta cttcctcatt
       61 tccaagttcc tggccgcggg accgctggag gagacggcga aggtcttgat ccgggaactc
      121 gaagagaaga aggttttgcc gcgtcgtctc gattggcagg gaaacgagca tgagcagacc
      181 tttgaggaat tggaacgaaa gtacaagcac attggcgcca atcacttgct tgaaatatgc
      241 agtcgtctgg gtccactggt ggatcgcgag cttccgccca gtgttccggg catcaactcg
      301 ctgttgggta ctgggcgcca gaacctcctg cgcaccaagg aaaccgtcta ctgccatcgc
      361 tccttaaggg actactgcac ccgcctgaat ggagtctcgc tgccggattc ggtccttaca
      421 aagccgaccc acaacttaga tcgcgttcta actggacggg aacacggcgg ggaagtccgt
      481 cgcaagctcc tcgtgcccac ggacctctac cgccgaacaa aattgctccg tcgcactgtg
      541 ggccacctgt cctcagtgta ctgtgttctg tttgatcgca caggaagata catcatcacc
      601 ggagccgatg atctacttat taagatatgg tcagcggcag atggccgcct gctggccact
      661 ctgcgaggcg cctcatccga gattacagac atagccatta acttggacaa tacgatgctg
      721 gctgccgggt cattggatca catactaaga gtgtgggaca tgcaaaccac atcacccatc
      781 gccgtactgt ctgctcacac gggcatgata acttcggtta acttttgtcc gtcgccacgt
      841 agcgatctga aatatctggt gactaccagc acagatggat cgatcgcctt ctggcaatac
      901 tcaacgccgc gcgggcaaaa gatcacgttt gccccgaagc caacgcagta tcatgagaaa
      961 ctgcggcccg gacaagctca gatgatgtgt accacctttt cgccaggagg catttttctg
     1021 gccgctgggt ctgccgatca ccatgtgcgc gtctatatga tgggcgagga cggtccgaag
     1081 cgaatattgg agacggaagc ttacacagat gccgtggact cggtgcagtg gtcgcatcgt
     1141 ggactgagat ttatatcggg gagcaaggac ggcacagcac acatctggac gtttgagtcg
     1201 cagcagtgga aaagttccaa actatgcatg acggagcgtc tggccagctg cccggaacca
     1261 gaggagggaa agaggcttaa ggtaaccatg gtggcttggg atgcctcgga ccgttatgtc
     1321 atcaccgccg taaacgattt tactataaaa atctgggact cgaagtcagc caagctgcat
     1381 cgtgttctgc gtgggcacaa ggatgagctt tatgtgcttg agtcgaaccc aagagatgag
     1441 catgtcctgc tttccgccgg gcacgatggc caggtatttc tgtgggacat cgagcagggc
     1501 gtttgtgtgg ccaacttcct taacgacatc gatggccaag gccatggcag tgtctttgat
     1561 gcaaagtggt caccggatgg cacaatgata gctgccaccg attcgcatgg acacatcctg
     1621 atttttggtt tgggcgtgtg cattgaaaag tacaaaatgc ttcccacgga gctgttcttt
     1681 cacacggatt ttcgaccttt attgagagat gcgcagcacc atgtggtgga cgagcagacg
     1741 cagataatgc cgcacctaat gcccccgcct ttcctggtcg atgcagatgg caacccgcat
     1801 ccatcaaggt ttcaacgatt tgtgcctggt cgtgagagct gtagcctaga ccaattgatt
     1861 ccaaatctta cggtaggcgt ggatggcgta gtaatcgacg atggaggtgc aggtggagat
     1921 gctgttgtgg ccgctgcacc cgctgaagct ccgccagccg cattgccccc tgctgctgct
     1981 ggaggaccac agggagctaa ctactcccat atcgaccgca tgattgcagc cttggccaat
     2041 cggcagtcgg ttaatgtaaa tgccaatgat gccaatccga ccaacggcaa ccagtccttc
     2101 gaacgtttga ccaatcgtca aatgccggac tccaatggca ggcaatcgtc acgtggcaat
     2161 gtcctgggta gtcgtttgag cacgcctcgc caaggatggg gtgccgcagc tgccgcgcca
     2221 gttgaggatc aagcagggcc gtctcaacct ccggcagaac ctgcagccga acctggccag
     2281 gcacaagtcc aacaggcgct gcctctgaaa ttcatccgtc gcacttatgt gcgtcccatg
     2341 aagtatcccc agttgcaaaa ccttaagcag actatttact ctgccggtca gtttgaaaag
     2401 caggagtaca aacgcgaaat gcgacgccga cccatcatga taaacactgc cagtgcagca
     2461 tcttcgcagc agccaggaag cgttggcagg ccacgaaaca cacgtggcaa tggcggcggc
     2521 gcaagagctg gacgaagacg tggcccccag ccagctggcg gacagccaca acccgcttat
     2581 cgcacccgcg ctgttcggga tcaggagccg gagcactacg atgaggtagc gccgccaccc
     2641 gaggaagagg aggaagatgt gtccagcaac tcgtccggcg acacgagcta ctcgaatgtg
     2701 gaagaaaacc tagaggacag cagcgatgag tcggaaacgg acagttccga ttactctgac
     2761 tgggtggcgg atactcctgg cccgaatctg gaaccaccaa aacgatccaa gcgtaagcca
     2821 ctctcacggc gaccccggag tagtgatgac agcagcgatg atgagcgagc gggcagatcc
     2881 agaggtgggc aggttacggc ccggaaggtt ggacgtaaaa ctgttttgtt tccgcccaca
     2941 gatgcaaatg gagagatacc ggagctttat cgtcccgctg agtggctatc tgaggtgatt
     3001 ccacgtaagg caccctatta cccccagatg ggtgatgagg tagtctactt ccggcaagga
     3061 cacgcaaagt acttggatgc agtccgcctg aaaaaggtct acaagctctc gcacagctcg
     3121 gagccgtgga actttcacac actgcgcgac cacgagctgg tgcgtgtgat tggaatcaag
     3181 tacgaaatcc gcccaccccg attatgttgc ttgaagttgg ccatcatcga tgaggagggc
     3241 aacatgacgg ggacaacgtt caagatcaag taccatgaca tgcccgacgt tttggacttt
     3301 ttagtgctgc ggcagacctt cgatcttgca gtgcagcgca actggagcat tggtgaccgg
     3361 tttcgctgca tgattgtcga tggctggtgg atgggtcaga ttgaatcgcg ccacgccctc
     3421 tctgctgact tccccgactc gtccttcatg tgtttccgcg tgcgctggga taatggagag
     3481 tatgagttta tgagtccctg ggacatggaa cccatcgatc agcaaagagt accggacgag
     3541 gtgggaggtg ctgttccagt tctaccggag gagattcgcg ccactctata ccagcccaag
     3601 tccgaggaat ggcacagagg ggatcgcgat ggcacctgca ggcgaattat caatggattg
     3661 gagcaggtta tgcgtctctc tatcgcagag catttcctgg cccccgtgga ccttaacata
     3721 tatccagact acgcttattt gatcgaatat cccattgacc tgaccacaat taaatctcgc
     3781 tttgagaatc acttctaccg cagaataacc tcagcccagt tcgatgtacg ctacctagcc
     3841 accaatgcgg agcagtacaa tcgccgacac gcaattatcg tcaagcattc gcgaattgtg
     3901 acagatcttt gtttgaggat catcagggaa ccggatgaga tagatgtggc tgctgtttac
     3961 catcagcttg cggatgtgta ccattcgtcc gagtcggaga acgaggtaga ttcggatgta
     4021 gtgccatcga ccagcacagg gccaactact tcagcagctg cagcagcagc taggcaaaga
     4081 gtatcctcag ccaggcgatc cactcgaatt cactcggaag gagactggcg tgccgattgt
     4141 cgtcagctgc ttgatcttat gtgggcgagg acagattcgg cgccgttccg cgagcccgtt
     4201 gacaccatcg acttccctga ctacctggag atcatagcta ctccaatgga tctgcgcact
     4261 gtaaaggagg atctgctggg cggcaactac gatgatccgt tggactttgc caaggatgtg
     4321 cggctgatct tccaaaactc caagaactac aacaccaaca agcgctcacg gatttatgcc
     4381 atgaccttgc gactcagtgc gctatttgag tcgcatatca agacagtggt caccagctgg
     4441 aaggcggcat taaggcgcgc taataaaaac aagtccggca gcagtggagg aggacgcggt
     4501 ggttccagca gcagtcccaa caagcgagtc aaaaaggagc gatccacgcg ccgtaatcaa
     4561 aagccaacag cagcggccag aagattgcca gtcgccaact ccagtgaaga tgaggacgag
     4621 gaggatgatg atgatgacga cgatgatgat gatgaggaag aggcatcaac gagggggcgc
     4681 tctcgtcggc gaaacggtgt ggccgtaagt gctagttcct ctggctccaa tcgcgtgact
     4741 tcttcatccg ccgcccaaca gcgtcgaggc aaaagcagca gtagcgagga ggaagaggag
     4801 gaggaaacca gtgcgcaggc cacgtcggat gccagcagcg atgaagagga aagagcccaa
     4861 tccaatgaca gttcgcccca gcagacacgc acgcgcaggg caaggcagcg gcaacggctg
     4921 agcgatgact ccaatatcaa cgattccgag gatagttaca accccaacga gggacgcggc
     4981 agtcggcgac gtccaggtgc cgcacgcccg tccagcaata ggcagacaaa tggccactcc
     5041 agcaagaggc gacgtttgaa caaaagtcca gccaatgctg cacctggccc tagtttgcca
     5101 cgtcggacac gtcgcatagc aggcagctcc gcagaagagg ctgcatctca ctcacaacac
     5161 gatgagagca cacaggatag ccagatagga cgtcgaaagg gacgctcggc aagcagcagg
     5221 tcaattggca atggaccaag taccagtgcc gcagctgccg ccaactccag ccttgaaagt
     5281 ccgtcacgga atactcgagc caacggaacc aggacctctt cgcatcttgg agccgaaaat
     5341 gatcacagtt accacctgcc agtgcgcaac gggcgcatta tcgagtccga tacggactct
     5401 cctgtcatca gacccacgcg acagagcgcc aagagggcgc taatggactg gggtcgtgga
     5461 agcgatattg aagaggagga ggaggaagag gaagaagaca gcgaggaagc cgaggagata
     5521 ctgccaacac ctacaaagga cgacttgccc tctaccagcc gagccgcttt ggctcaggcg
     5581 gctgctggtc cttctcgcca gctacgcacg aaccgcaaca ccgtcgttcg tccagcccag
     5641 agtgacgacg atgacgacga cgacagtgac aacgagccgc tggccaacaa tcaacaaaaa
     5701 aattatcaat cacctgcaaa atctagctca accgcaacag tggcccctca tccactgagc
     5761 ctgcgccgtc gattagcctc gccgccagaa cggaataccc atatgacccg ttcccatgcc
     5821 acctccacaa cgtcctcggc cgcagcatca cgggcagaac aacaaccagc ggtttccacc
     5881 cacgatcaca actacctagg caacgtgaat tcatcgaggg caccgcgccg cattctttca
     5941 cgccaccaac gaaatgccga cgagttggat accagtctag atgtcctgga aaactcgcgt
     6001 ctgctctcag ccatagccaa tcaccggcga cccacctcta ccacgaacag caacagtgcg
     6061 agcaacgtgg ttacaaggcg tctgcgtggt cgcgctagcc aggcgaattc gcacgacgag
     6121 gatcatgagg aagaggaggc tgctgcttcc gatgagggca ctgatggagg ctctagctcg
     6181 cagagctacg aagatgagga tgaagactcc gactcggaag acaatcagcc gctgaccagc
     6241 tacgtatcgc ccagcaatgg tcgtccgagg cgaaaggcaa aaacctcctc ctcctccgca
     6301 acaactcagg agcgtccgct gcgacgcaca cgtcgcccgc catccaataa ctccatcgcg
     6361 aacgacaacg atgacgatga tgactatgtt gtgccgcgga gtcgtcgcgc caaccggggt
     6421 ggaaaccagc gagccgggcg caaccaaaag agaccgcgct acaatgagca gtccgaggag
     6481 gaagaggcgg cggctggata ttcccatcgg agtaaacgaa tccgtaacgg cgacgctcat
     6541 gccagctctc aggagcgatc acaggcctcc accgagcggg accaggagga agaagaggac
     6601 ggtacttcgg tggactcgga agaacagcag gtgaacgtta gcagcagagg cagggtgcgc
     6661 aagccgtctg ccaaggcgcg cggtatcttc aaggagtggc ggccgctcga gtctagaggg
     6721 cccttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     6781 catcatcacc atcaccattg agttt
//
