LOCUS       sequence-70394-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PesaR promoter, luxCDABE, and terminator in pET17b (AmpR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pET-PesaRlux
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(174..278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     346..364
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      301 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
      361 aggccctttc gtcttcacct cgaggcagat tgagtaaccg tgaatgtttg tacaaatgtt
      421 tcaaagatgt tactatgagt gtcccggcca gcatcacttt atattttgtg acgtctggcc
      481 ggacgttttc cctagtgttg gctgttttag cgacctggcc gtacaggtca ggtttttttt
      541 taccgctaaa caactgaagc cattgtaacc tctgaatgat tcattgtaag ttactcttaa
      601 gtatcatctt gcctgtacta tagtgcaggt taagtccacg ttaagtaaaa gaagcagcgg
      661 atcctctagt tgcggccgca aatggatggc aaatatgact aaaaaaattt cattcattat
      721 taacggccag gttgaaatct ttcccgaaag tgatgattta gtgcaatcca ttaattttgg
//
