LOCUS       sequence-70416-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  esaI gene downstream of esaR-a272g in pAC-EsaR-D91G (CmR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pAC-EsaR-D91G-EsaI
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(145..150,151..168,169..175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    183..199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     188..210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             217..228
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttctagacca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
       61 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      121 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      181 aattgtgagc ggataacaat ttcacacagg aaacataaag aggagaaagg tacccatgat
      241 gttctctttc ttccttgaaa accaaacaat aacggatacg cttcagactt acatacagag
      301 aaagttatct ccgctgggta gtccggatta cgcttacact gttgtgagca aaaaaaatcc
      361 ttcaaatgtt ctgattattt ccagttatcc tgacgaatgg attaggttat accgcgctaa
      421 caactttcag ctgaccgatc ccgttattct cacggccttt aaacgcacct cgccgtttgc
      481 ctgggatgag aatattacgc tgatgtccgg cctgcggttc accaaaattt tctctttatc
      541 caagcaatac aacatcgtta acggctttac ctatgtcctg catgaccaca tgaacaacct
      601 tgctctgttg tccgtgatca ttaaaggcaa cgatcagact gcgctggagc aacgccttgc
      661 tgccgaacag ggcacgatgc agatgctgct gattgatttt aacgagcaga tgtaccgact
      721 ggcaggcacc gaaggcgagc gagccccggc gttaaatcag agcgcggaca aaacgatatt
      781 ttcctcgcgt gaaaatgagg tgttgtactg ggcgagtatg ggcaaaacct a
//
