LOCUS       sequence-70430-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xHA-tagged 
            constitutively active MKK6 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo 
            Z, Yang D, Luo H, McManus BM, Janes KA
  TITLE     An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis 
            during coxsackievirus B3 infection.
  JOURNAL   Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 
            10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.
  PUBMED    23332156
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2012.11.009"; journalName: "Cell Host Microbe"; 
            date: "2013-01-16- 16"; volume: "13"; issue: "1"; pages: "67-76"
COMMENT     Sequence Label: pSLIK 3xHA-MKK6-EE neo
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        141..455
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    149..167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    185..203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    292..310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     428..452
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             467..484
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             488..568
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
       61 ttggttaatt ctagacagtt atcaacaagt ttgtacaaaa aagcaggctt taaaggaacc
      121 aattcagtcg actggatccc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      181 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      301 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
      421 taagcagagc tcgtttagtg aaccgtcaga tcgccactag tcggatctgg ttccgcgtgg
      481 atctatgtac ccatacgatg ttccagatta cgcttaccca tacgatgttc cagattacgc
      541 ttacccatac gatgttccag attacgctgg atctatgtct cagtcgaaag gcaagaagcg
      601 aaaccctggc cttaaaattc caaaagaagc atttgaacaa cctcagacca gttccacacc
      661 acctagagat ttagactcca aggcttgcat ttctattgga aatcagaact ttgaggtgaa
      721 ggcagatgac ctggagccta taatggaact gggacgaggt gcgtacgggg tggtggagaa
      781 gatgcggcac gtgccc
//
