LOCUS       sequence-70435-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible 3xHA-tagged 
            constitutively active MKK6 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo 
            Z, Yang D, Luo H, McManus BM, Janes KA
  TITLE     An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis 
            during coxsackievirus B3 infection.
  JOURNAL   Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 
            10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.
  PUBMED    23332156
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chom.2012.11.009"; journalName: "Cell Host Microbe"; 
            date: "2013-01-16- 16"; volume: "13"; issue: "1"; pages: "67-76"
COMMENT     Sequence Label: pSLIK 3xHA-MKK6-EE neo
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(170..787,788..793,794..829,830..832,833..868,869..871,
                     872..907,908..916)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
       61 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
      121 taccgagctc ggatctccac cccgtaccgg tcctgcagtc gaattcacca tgtctagact
      181 ggacaagagc aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg
      241 cctgacgaca aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca
      301 cgtgaagaac aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca
      361 tacccacttc tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa
      421 gtcataccgc tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
      481 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
      541 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
      601 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
      661 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
      721 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
      781 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
      841 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
      901 catgctcccc gggtaactaa
//
