LOCUS       sequence-70438-e        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, 
            Sabatini DM
  TITLE     mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms 
            define three distinct mTORC2s.
  JOURNAL   Curr Biol. 2006 Sep 19;16(18):1865-70. doi: 
            10.1016/j.cub.2006.08.001. Epub 2006 Aug 17.
  PUBMED    16919458
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2006.08.001"; journalName: "Curr Biol"; date: 
            "2006-09-19- 19"; volume: "16"; issue: "18"; pages: "1865-70"
COMMENT     Sequence Label: pMSCV-TAP
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..26
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(118..195)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             complement(202..222)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(259..432)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(433..606)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(655..1071)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(671..687)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(703..725)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
       61 cggtagaatt cccggcggcc gcgggatcca ttcgctcgag ggaccaccac caccaccaag
      121 tgccccggag gatgagattt tcttaaagcg gttggctgct gagacggcta tgaaattctt
      181 tttccatctt ctctttagtt caccttgaaa atataaattt tcagaagcag ttgtaggtat
      241 atcacaattc gcgtctactt tcggcgcctg agcatcattt agctttttag cttctgctaa
      301 aaggttagcg ctttggcttg ggtcatcttt taaactttgg atgaaggcgt ttcgttgttc
      361 ttcgtttaag ttaggtaaat gtaagatctc atagaacgcg ttttgttgtt ctttgttgaa
      421 tttgttgtct actttcggcg cctgagcatc atttagcttt ttagcttctg ctaaaaggtt
      481 agcgctttgg cttgggtcat cttttaaact ttggatgaag gcgtttcgtt gttcttcgtt
      541 taagttaggt aaatgtaaga tctcatagaa cgcgttttgt tgttctttgt tgaatttgtt
      601 gtccacggct tcatcgtgtt gcgcaaggcc tgccatggtg cctgatctaa ttccggcgcc
      661 tagagaagga gtgagggctg gataaaggga ggatcgaggc ggggtcgaac gaggaggttc
      721 aagggggaga gacggggcgg atggaggaag aggaggcgga ggcttagggt gtacaaaggg
      781 cttgacccag ggaggggggt caaaagccaa ggcttcccag gtcacgatgt aggggacctg
      841 gtctgggtgt ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc
      901 tcggttaaag gtgccgtctc gcggccatcc gacgttaaag gttggccatt ctgcagagca
      961 gaaggtaacc caacgtctct tcttgacatc taccgactgg ttgtgagcga tccgctcgac
     1021 atctttccag tgacctaagg tcaaacttaa gggagtggta acagtctggc cctaatttt
//
