LOCUS       sequence-70439-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a c659t mutation in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-V220A
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    114..135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(150..155,156..173,174..180)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    188..204
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     193..215
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             222..233
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 aatatttcta gaccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
       61 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      121 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      181 tgtggaattg tgagcggata acaatttcac acaggaaaca taaagaggag aaaggtaccc
      241 atgatgttct ctttcttcct tgaaaaccaa acaataacgg atacgcttca gacttacata
      301 cagagaaagt tatctccgct gggtagtccg gattacgctt acactgttgt gagcaaaaaa
      361 aatccttcaa atgttctgat tatttccagt tatcctgacg aatggattag gttataccgc
      421 gctaacaact ttcagctgac cgatccggtt attctcacgg cctttaaacg cacctcgccg
      481 tttgcctggg atgagaatat tacgctgatg tccgacctgc ggttcaccaa aattttctct
      541 ttatccaagc aatacaacat cgttaacggc tttacctatg tcctgcatga ccacatgaac
      601 aaccttgctc tgttgtccgt gatcattaaa ggcaacgatc agactgcgct ggagcaacgc
      661 cttgctgccg aacagggcac gatgcagatg ctgctgattg attttaacga gcagatgtac
      721 cgactggcag gcaccgaagg cgagcgagcc ccggcgttaa atcagagcgc ggacaaaacg
      781 atattttcct cgcgtgaaaa tgaggtgttg tactg
//
