LOCUS       sequence-70442-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a208g, a272g, c659t mutations in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-I70V/D91G/V220A
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    112..133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(148..153,154..171,172..178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     191..213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             220..231
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tatttctaga ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
       61 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      181 tggaattgtg agcggataac aatttcacac aggaaacata aagaggagaa aggtacccat
      241 gatgttctct ttcttccttg aaaaccaaac aataacggat acgcttcaga cttacataca
      301 gagaaagtta tctccgctgg gtagtccgga ttacgcttac actgttgtga gcaaaaaaaa
      361 tccttcaaat gttctgatta tttccagtta tcctgacgaa tggattaggt tataccgcgc
      421 taacaacttt cagctgaccg atccggttgt tctcacggcc tttaaacgca cctcgccgtt
      481 tgcctgggat gagaatatta cgctgatgtc cggcctgcgg ttcaccaaaa ttttctcttt
      541 atccaagcaa tacaacatcg ttaacggctt tacctatgtc ctgcatgacc acatgaacaa
      601 ccttgctctg ttgtccgtga tcattaaagg caacgatcag actgcgctgg agcaacgcct
      661 tgctgccgaa cagggcacga tgcagatgct gctgattgat tttaacgagc agatgtaccg
      721 actggcaggc accgaaggcg agcgagcccc ggcgttaaat cagagcgcgg acaaaacgat
      781 attttcctcg cgtgaaaatg aggtgttgta
//
