LOCUS       sequence-70452-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PesaR-D promoter, luxCDABE, and terminator in pET17b (AmpR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pET-PesaR-Dlux
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(185..289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     357..375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
       61 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      361 ggcgtatcac gaggcccttt cgtcttcacc tcgaggcaga ttgagtaacc gtgaatgttt
      421 gtacaaatgt ttcaaagatg ttactatgag tgtcccggcc agcatcactt tatattttgt
      481 gaagtctggc cggacgtttt ccctagtgtt ggctgtttta gcgacctggc cgtacaggtc
      541 aggttttttt ttaccgctaa acaactgaag ccatgcctgt actatagtgc aggtattgta
      601 agttactctt aagtatcatc ttgcctgtac tatagtgcag gttaagtcca cgttaagtaa
      661 aagaagcagc ggatcctcta gttgcggccg caaatggatg gcaaatatga ctaaaaaaat
      721 ttcattcatt attaacggcc aggttgaaat ctttcccgaa agtgatgatt tagtgcaatc
      781 cattaatttt
//
