LOCUS       sequence-70454-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PesaR-P promoter, luxCDABE, and terminator in pET17b (AmpR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pET-PesaR-Plux
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     347..365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
       61 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      301 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
      361 gaggcccttt cgtcttcacc tcgaggcaga ttgagtaacc gtgaatgttt gtacaaatgt
      421 ttcaaagatg ttactatgag tgtcccggcc agcatcactt tatattttgt gaagtctggc
      481 cggacgtttt ccctagtgtt ggctgtttta gcgacctggc cgtacaggtc aggttttttt
      541 ttaccgctaa acaactgaag ccattgtaac ctctgaatga ttcattgtaa gttactctta
      601 agtatcatct tgcctgtact atagtgcagg ttaagtccag cctgtactat agtgcaggtg
      661 gatcctctag ttgcggccgc aaatggatgg caaatatgac taaaaaaatt tcattcatta
      721 ttaacggcca ggttgaaatc tttcccgaaa gtgatgattt agtgcaatcc attaattttg
      781 gtgataatag tgtttacctg ccaatatt
//
