LOCUS       sequence-70456-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a208g and a272g mutations in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-I70V/D91G
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(146..151,152..169,170..176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    184..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     189..211
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             218..229
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tttctagacc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
       61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      181 gaattgtgag cggataacaa tttcacacag gaaacataaa gaggagaaag gtacccatga
      241 tgttctcttt cttccttgaa aaccaaacaa taacggatac gcttcagact tacatacaga
      301 gaaagttatc tccgctgggt agtccggatt acgcttacac tgttgtgagc aaaaaaaatc
      361 cttcaaatgt tctgattatt tccagttatc ctgacgaatg gattaggtta taccgcgcta
      421 acaactttca gctgaccgat ccggttgttc tcacggcctt taaacgcacc tcgccgtttg
      481 cctgggatga gaatattacg ctgatgtccg gcctgcggtt caccaaaatt ttctctttat
      541 ccaagcaata caacatcgtt aacggcttta cctatgtcct gcatgaccac atgaacaacc
      601 ttgctctgtt gtccgtgatc attaaaggca acgatcagac tgcgctggag caacgccttg
      661 ctgccgaaca gggcacgatg cagatgctgc tgattgattt taacgagcag atgtaccgac
      721 tggcaggcac cgaaggcgag cgagccccgg cgttaaatca gagcgcggac aaaacgatat
      781 tttcctcgcg tgaaaatgag
//
