LOCUS       sequence-70457-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a208g mutation in esaR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-I70V
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..132
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(147..152,153..170,171..177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    185..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     190..212
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             219..230
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atttctagac caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
       61 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
      121 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
      181 ggaattgtga gcggataaca atttcacaca ggaaacataa agaggagaaa ggtacccatg
      241 atgttctctt tcttccttga aaaccaaaca ataacggata cgcttcagac ttacatacag
      301 agaaagttat ctccgctggg tagtccggat tacgcttaca ctgttgtgag caaaaaaaat
      361 ccttcaaatg ttctgattat ttccagttat cctgacgaat ggattaggtt ataccgcgct
      421 aacaactttc agctgaccga tccggttgtt ctcacggcct ttaaacgcac ctcgccgttt
      481 gcctgggatg agaatattac gctgatgtcc gacctgcggt tcaccaaaat tttctcttta
      541 tccaagcaat acaacatcgt taacggcttt acctatgtcc tgcatgacca catgaacaac
      601 cttgctctgt tgtccgtgat cattaaaggc aacgatcaga ctgcgctgga gcaacgcctt
      661 gctgccgaac agggcacgat gcagatgctg ctgattgatt ttaacgagca gatgtaccgc
      721 ctggcaggca ccgaaggtga acgagccccg gcgttaaatc agagcgcgga caaaacgata
      781 ttttcctcgc gtgaaaatga ggtgttgtac tgggcgagta tgggcaaaac
//
