LOCUS       sequence-70466-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, 
            Devenney PS, Hohenstein P
  TITLE     A universal vector for high-efficiency multi-fragment recombineering
            of BACs and knock-in constructs.
  JOURNAL   PLoS One. 2013 Apr 29;8(4):e62054. doi: 
            10.1371/journal.pone.0062054. Print 2013.
  PUBMED    23637962
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-04-29"; volume: "8(4):e62054. doi"; pages: " 
            10.1371/journal.pone.0062054. Print 2013"
COMMENT     Sequence Label: pDEST-IRES-puro-polyA
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..81
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     66..82
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    98..222
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(753..772)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga catatgagat ctgttttaaa cacgcgtgct
       61 agcatcgagg tcgacggtat cgataagctt gatatcaaca agtttgtaca aaaaagctga
      121 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      181 gactacataa tactgtaaaa cacaacatat ccagtcacta tgaatcaact acttagatgg
      241 tattagtgac ctgtagtcga ccgacagcct tccaaatgtt cttcgggtga tgctgccaac
      301 ttagtcgacc gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac
      361 tcgctattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag
      421 cctctttttt gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt
      481 cctgaaaatc atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc
      541 gttcggcttc atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa
      601 tttctactgt atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      661 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      721 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      781 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
      841 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
      901 tcc
//
