LOCUS       sequence-70469-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     Fritjof Helmchen Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-EF1a-YCNano140
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..781)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     primer_bind     240..259
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     499..520
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(623..644)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(788..865)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttcttactcg atgttgtggc
       61 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
      121 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      181 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      241 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      301 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca
      361 ggccgtagcc cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      421 agatcagctt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      481 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      541 acagctcctc gcccttgctc accatgccac cgctgccacc cttgtacagc tcgtccatgc
      601 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      661 tggggtcttt gctcagggcg gactggtagc tcaggtagtg gttgtcgggc agcagcacgg
      721 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ccgccgtcca
      781 tgagctccag tgccccggag ctggagatct tcttgaaccg gttggcagcg ctgacggcaa
      841 tgaagttttt cttccagcgc ctcttgg
//
