LOCUS       sequence-70473-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector for Arabidopsis transformation with BsaI Golden Gate 
            sites and mCherry seed selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Emami S, Yee MC, Dinneny JR
  TITLE     A robust family of Golden Gate Agrobacterium vectors for plant 
            synthetic biology.
  JOURNAL   Front Plant Sci. 2013 Sep 2;4:339. doi: 10.3389/fpls.2013.00339. 
            eCollection 2013.
  PUBMED    24032037
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2013-09-2"; volume: "4:339. doi"; pages: " 
            10.3389/fpls.2013.00339. eCollection 2013"
COMMENT     Sequence Label: pGoldenGate-SE7
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      266..518
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(616..939)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     621..640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     834..856
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     848..865
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     849..865
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    866..922
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(935..951)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(935..951)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(948..970)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    959..975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg aggagctgta
      241 caagtaaagc ggccgcccgg ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
      301 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
      361 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
      421 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
      481 taaattatcg cgcgcggtgt catctatgtt actagatcgg ggcgcgccct cgagagtaag
      541 agacccggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      601 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
      661 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
      721 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      781 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      841 acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat cctctagagt
      901 cgacctgcag gcatgcaggc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      961 gttatccgct cacaattccc acacaacata cgagccggaa gcat
//
