LOCUS       sequence-70484-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector for Arabidopsis transformation with SapI Golden Gate 
            sites and mCherry seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Emami S, Yee MC, Dinneny JR
  TITLE     A robust family of Golden Gate Agrobacterium vectors for plant 
            synthetic biology.
  JOURNAL   Front Plant Sci. 2013 Sep 2;4:339. doi: 10.3389/fpls.2013.00339. 
            eCollection 2013.
  PUBMED    24032037
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2013-09-2"; volume: "4:339. doi"; pages: " 
            10.3389/fpls.2013.00339. eCollection 2013"
COMMENT     Sequence Label: pGoldenGate-MCY2
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    36..212
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     314..331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    448..469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(484..489,490..507,508..514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    522..538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     527..549
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     546..562
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     546..562
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             558..881
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    575..631
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(632..649)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(632..648)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(641..663)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(857..876)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcggccgctc tagaactagt ccgctctagc ccatcgatct ggattttagt actggatttt
       61 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
      121 gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat ttcccttatc
      181 gggaaactac tcacacatta tttatggaga aaatagagag agatagattt gtagagagag
      241 actggtgatt tcagcgtacc gacaagcttc catgaagagc gataccgctc gccgcagccg
      301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      421 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
      541 tcacacagga aacagctatg accatgatta cgccaagcct gcatgcctgc aggtcgactc
      601 tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
      661 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      721 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      781 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      841 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      901 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct ccc
//
