LOCUS       sequence-70486-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector for Arabidopsis transformation with BsaI Golden Gate 
            sites and Kanamycin selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Emami S, Yee MC, Dinneny JR
  TITLE     A robust family of Golden Gate Agrobacterium vectors for plant 
            synthetic biology.
  JOURNAL   Front Plant Sci. 2013 Sep 2;4:339. doi: 10.3389/fpls.2013.00339. 
            eCollection 2013.
  PUBMED    24032037
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2013-09-2"; volume: "4:339. doi"; pages: " 
            10.3389/fpls.2013.00339. eCollection 2013"
COMMENT     Sequence Label: pGoldenGate-SE9
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..209)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(62..241)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(403..726)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     408..427
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     621..643
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     635..652
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     636..652
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    653..709
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(722..738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(722..738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(735..757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    746..762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(770..776,777..794,795..800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    815..836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atcatgcgaa acgatccaga tccggtgcag attatttgga ttgagagtga atatgagact
       61 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
      121 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
      181 gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt
      241 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac
      301 tcccttaatt ctccgctcga gagtaagaga cccggatgcc gggagcagac aagcccgtca
      361 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
      421 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
      481 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
      541 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
      601 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
      661 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaggcttg gcgtaatcat
      721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      901 tcggccaacg cgcggg
//
