LOCUS       sequence-70488-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector for Arabidopsis transformation with SapI Golden Gate 
            sites and mCherry seed selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Emami S, Yee MC, Dinneny JR
  TITLE     A robust family of Golden Gate Agrobacterium vectors for plant 
            synthetic biology.
  JOURNAL   Front Plant Sci. 2013 Sep 2;4:339. doi: 10.3389/fpls.2013.00339. 
            eCollection 2013.
  PUBMED    24032037
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2013-09-2"; volume: "4:339. doi"; pages: " 
            10.3389/fpls.2013.00339. eCollection 2013"
COMMENT     Sequence Label: pGoldenGate-MCY2
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      263..515
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(613..936)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     618..637
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     831..853
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     845..862
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     846..862
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    863..919
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(932..948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(932..948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(945..967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    956..972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggagg agctgtacaa
      241 gtaaagcggc cgcccggctg cagatcgttc aaacatttgg caataaagtt tcttaagatt
      301 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      361 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      421 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      481 attatcgcgc gcggtgtcat ctatgttact agatcggggc gcgccctcga gagtagaaga
      541 gccggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      601 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
      661 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
      721 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      781 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      841 acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga
      901 cctgcaggca tgcaggcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      961 atccgctcac aattcc
//
