LOCUS       sequence-70493-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target 
            site in the C. elegans genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD162 (Peft-3::Cas9 + Empty sgRNA)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..618
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 acttgtatag aaaagttggc acctttggtc ttttattgtc aacttccatt ggttcttcca
       61 ttgtttctgt taaattaatg aatttttcat aaaataaaga cattatacaa tataaaaatg
      121 aagaatttat tgaaaataaa ctgccagaga gaaaaagtat gcaacactcc cgccgagagt
      181 gtttgaaatg gtgtacggta cattttcgtg ctaggagtta gatgtgcagg cagcaacgag
      241 agggggagag atttttttgg gccttgtgaa attaacgtga gttttctggt catctgacta
      301 atcatgttgg ttttttgttg gtttattttg tttttatctt tgtttttatc cagattagga
      361 aatttaaatt ttatgaattt ataatgaggt caaacattca gtcccagcgt ttttcctgtt
      421 ctcactgttt agtcgaattt ttattttagg ctttcaacaa atgttctaac tgtcttattt
      481 gtgacctcac tttttatatt tttttaattt ttaaaaatat tagaagtttc taggataatt
      541 ttttcgactt ttattctctc taccgtccgc actcttctta cttttaaatt aaattgtttt
      601 tttttcagtt gggaaacact ttgctctaga aaaaatggac aaaaaataca gcatcggcct
      661 tgacatcgga acgaactccg tcggatgggc cgtcatcacc gacgagtaca aggtcccatc
      721 caagaagttc aaggtcctcg gaaacaccga ccgtcactcc atcaagaaga acctcatcgg
      781 agccctcctc ttcgactccg gagagaccgc cgaggccacc cgtctcaagc gtaccgcccg
      841 tcgtcgttac acccgtcgta agaaccgtat ctgctacctc caagagatct tctccaacga
      901 gatg
//
