LOCUS       sequence-70495-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pH sensor, targeted to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Poburko D, Santo-Domingo J, Demaurex N
  TITLE     Dynamic regulation of the mitochondrial proton gradient during 
            cytosolic calcium elevations.
  JOURNAL   J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 
            10.1074/jbc.M110.159962. Epub 2011 Jan 11.
  PUBMED    21224385
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.159962"; journalName: "J Biol Chem"; date: 
            "2011-04-1- 1"; volume: "286"; issue: "13"; pages: "11672-84"
COMMENT     Sequence Label: SypHer mt
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..93
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             115..186
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="SVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     825..846
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(955..976)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
       61 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttggggga tctgtccgtc
      121 ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc ggcggctccc agtgccgcgc
      181 gccaagatcc attcgttggg ggatccaccg gtcgccaccg agatggcaag ccagcagggc
      241 gagacgatgt ccggaccgct gcacattggt ttgattccca cagttggacc gtacctgcta
      301 ccgcatatta tccctatgct gcaccagacc tttccaaagc tggaaatgta tctgcatgaa
      361 gcacagaccc accagttact ggcgcaactg gacagcggca aactcgattg cgtgatcctc
      421 gcgctggtga aagagagcga agcattcatt gaagtgccgt tgtttgatga gccaatgttg
      481 ctggctatct atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg
      541 gcaggggaaa aactgctgat gctggaagat ggtcactctt tgcgcgatca ggcaatgtcc
      601 gccggctaca acagcgacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      661 gccaacttca agatccgcca caacgtcgag gacggcagcg tgcagctcgc cgaccactac
      721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      781 ttccagtccg tcctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      841 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaacgt ggatggcggt
      901 agcggtggca ccggcagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      961 gagctggacg gcgacgtaaa cggccacaag ttca
//
