LOCUS       sequence-70500-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that is targeted to a genomic site near the 
            ttTi5605 Mos1 insertion allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..604
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 gttggcacct ttggtctttt attgtcaact tccattggtt cttccattgt ttctgttaaa
       61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa
      121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt
      181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt
      241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt
      301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat
      361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc
      421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt
      481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat
      541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga
      601 aacactttgc tctagaaaaa atggacaaaa aatacagcat cggccttgac atcggaacga
      661 actccgtcgg atgggccgtc atcaccgacg agtacaaggt cccatccaag aagttcaagg
      721 tcctcggaaa caccgaccgt cactccatca agaagaacct catcggagcc ctcctcttcg
      781 actccggaga gaccgccgag gccacccgtc tcaagcgtac cgcccgtcgt cgttacaccc
      841 gtcgtaagaa ccgtatctgc tacctccaag agatcttctc caacgagatg gccaaggtcg
      901 acgactcctt cttccaccgt ctcgaggagt cctt
//
