LOCUS       sequence-70501-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1902
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(364..382,383..388,389..405,406..411,412..437)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    364..382
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    389..407
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             451..462
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcatgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccgacgtct agggataaca gggtaattac
      181 ggccccagaa ttcaaaagat cttaagtaag taagagtata cgtatatcgg ctaaaacgta
      241 ttaaggcgct tcggcgcctt tttttatggg ggtattttca tcccaatcca cacgtccaac
      301 gcacagcaaa caccacgtcg accctatcag ctgcgtgctt tctatgagtc gttgctgcat
      361 atctccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcacatc
      421 agcaggacgc actgaccttt ggaattcatt aaagaggaga aaggtaccat ggcgagtagc
      481 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
      541 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
      601 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
      661 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
      721 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
      781 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
      841 cgtggtacca acttcccgtc cgac
//
