LOCUS       sequence-70503-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that is targeted to a genomic site near the 
            ttTi5605 Mos1 insertion allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(10..333)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(348..374)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(375..395)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagttgtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
       61 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
      121 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
      181 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
      241 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
      301 gagaagaagg gaatgcttga aaggattttg catttatcac tagtttaggc gtagtctggg
      361 acgtcgtatg ggtagacctt acgcttcttc tttggtggtc cggatcctcc gtctcctccg
      421 agttgggaga ggtcgatacg ggtctcgtag agtccggtga tggattggtg gatgagggtg
      481 gcgtcgagga cctccttggt ggaggtgtaa cgcttacggt cgatggtggt gtcgaagtac
      541 ttgaaggcgg ctggggctcc gaggttggtg agggtgaaga ggtggatgat gttctcggct
      601 tgctcacgga ttggcttgtc acggtgcttg ttgtaggcgg agaggacctt gtcgaggttg
      661 gcgtcggcga ggatgacacg cttggagaac tcggagattt gctcgatgat ctcgtcgagg
      721 tagtgcttgt gttgctcgac gaagagttgc ttttgctcgt tgtcctctgg ggatcccttg
      781 agcttctcgt agtgggaggc gaggtagagg aagttgacgt acttggatgg gagggcgagc
      841 tcgtttccct tttggagctc tccggcggag gcgagcatac gcttacgtcc gttctcgagc
      901 tcgaag
//
